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Immunohistochemical detection of infected neurons as a rapid diagnosis of enterovirus 71 encephalomyelitis

โœ Scribed by K. T. Wong; K. B. Chua; S. K. Lam


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
219 KB
Volume
45
Category
Article
ISSN
0364-5134

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To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera fro