Expression of caspid protein VP1 for use
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Shin-Ru Shih; Yi-Shuane Li; Chiuan-Chian Chiou; Pin-Chau Suen; Tzou-Yien Lin; Lu
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Article
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2000
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John Wiley and Sons
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English
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To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a polyhistidine fusion protein. Western blotting experiments with sera fro