Immunohistochemical demonstration of increased UDP-glucuronyltransferase in putative preneoplastic liver foci

✍ Scribed by G. Fischer; A. Schauer; K. W. Bock; D. Ullrich; N. Katz

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 276 KB
• Volume: 70
• Category: Article
• ISSN: 0028-1042
• DOI: 10.1007/bf00401611

✦ Synopsis

dine (DAB, Sigma, Grade II) and 0.01% (V/V) H202 in 0.12 M cacodylate buffer (pH 5.45) for 30 min. With the method described above we were able to stain very few and under optimal circumstances even one single cell. From our experience best results in filling one single cell with its dendritic branching and the efferent axon were obtained, if a very clear single-cell recording had occurred indicating that the electrode tip was as close as possible to one single cell (Fig. 1 b). In some preparations we could even find afferent cells to the recorded and injected neuron which were filled retrogradely.