Immunohistochemical characterization of a stage-specific antigen during oogenesis and spermatogenesis recognized with monoclonal antibody

✍ Scribed by Masaaki Itoh; Junpei Kimura; Azuma Tsukise; Masaomi Okano

• Book ID: 102966128
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 886 KB
• Volume: 18
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1994.1115

✦ Synopsis

ABSTRACT

In order to detect and characterize novel molecules which function in oogenesis, immunohistochemical study using a monoclonal antibody (MAb) raised against oocytes of Xenopus laevis was carded out. The distribution of the detectable molecule with MAb X‐80, which specifically reacts with Xenopus previtellogenic oocytes; stages I and II (Dumont, 1972), in particular was analyzed. The hamster, mouse, chick, quail, Caenorhabditis elegans and Lilium longiflorum were also examined. Interestingly MAb X‐80 bound not only in Xenopus oocytes but in its secondary spermatocytes and spermatids. Furthermore, MAb X‐80 gave a similar staining pattern in the Lilium and other, although the stage when the positive reaction is detectable is different in the male and female germ cells.