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Immunohistochemical and immunocytochemical localization of γ-melanocyte stimulating hormone (γ-MSH)-like immunoreactivity in human and rat hypothalamus

✍ Scribed by Osamura, R.Yoshiyuki; Komatsu, Noriyuki; Watanabe, Keiichi; Nakai, Yoshikatsu; Tanaka, Issei; Imura, Hiroo

Book ID
123286507
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
928 KB
Volume
3
Category
Article
ISSN
0196-9781

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✍ Yoshikuni Yogi; Seiichi Hashida; Rolf Ekman; Toshiaki Setoguchi; Dr. Eiji Ishika 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 832 KB

A noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for p-melanocyte-stimulating hormone (@-MSH) was developed. p-MSH (1-12) was biotinylated, trapped onto an anti-e-MSH (1-12) IgG-coated polystyrene bead, eluted at pH 1 after washing to eliminate other biotinylated substances,