Immunohistochemical analysis of p53 in colorectal cancer regarding clinicopathological correlation and prognostic significance

✍ Scribed by Hirotsugu Tomoda; Yoshihiro Kakeji

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 353 KB
• Volume: 58
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930580211

✦ Synopsis

The overexpression of the tumor suppressor gene p53 was investigated immunohistochemically in 144 cases of primary colorectal cancer and in 8 cases with cancer in the corresponding metastatic lymph nodes. Abnormalities in p53 expression were found in 36 cases (25%) of the 144 primary cancer cases. In addition, p53-positive tumors were found to metastasize frequently to the lymph nodes, as compared to p53-negative tumors (61.1% vs. 41.7%, P = 0.0428). p53 staining was identical in 7 of 8 (87.5%) cases in primary and metastatic lesions. When the DNA content of the tumor was determined by flow cytometry, the DNA index (mean k SD) was significantly higher in p53-positive tumors than in p53negative tumors (1.57 * 0.38 vs. 1.39 0.37, P = 0.012). Therefore, the immunohistochemical data of p53 in colorectal cancer may help in potentially predicting metastatic spread to the lymph nodes.