Immunoglobulins and α1-acid glycoprotein do not contribute to the cholesterol crystallization—promoting effect of concanavalin a—binding biliary protein
✍ Scribed by Marianne A. C.; De Bruijn; Kam S. Mok; Tiny Out; Guido N. J. Tytgat; Albert K. Groen
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 717 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
Human bile contains cholesterol crystallizationstimulating proteins that can be isolated by concanavalin A-Sepharose chromatography. In the past few years an increasing number of different pronucleating proteins have been identified in the concanavalin A-binding fraction. In this study we attempted to estimate the relative contribution of a number of these proteins to total concanavalin A-binding pronucleating activity. For this purpose, concanavalin Abinding glycoproteins were isolated from gallbladder bile samples from 12 patients with gallstones. The role of IgA, IgG and IgM and a,-acid glycoprotein was investigated by means of immunoextraction. No decrease in crystallization-promoting activity was observed after precipitation of more than 98% of the different immunoglobulins. In addition, removal of more than 95% of a,-acid glycoprotein from different concanavalin A-binding fractions had no significant effect on cholesterol crystallization-promoting activity. The influence of fibronectin was estimated by addition of physiological concentrations to a model bile system. At these concentrations fibronectin did not promote crystallization. From these data we conclude that immunoglobulins, a,-acid glycoprotein and probably also fibronectin do not significantly contribute to total concanavalin A-binding activity.