Immunoglobulin g lymphocytotoxic antibodies in clinical liver transplantation: Studies toward further defining their significance
✍ Scribed by Rafael Mañez; Robert H. Kelly; Makoto Kobayashi; Shunichi Takaya; Oscar Bronsther; David Kramer; Rene J. Duquesnoy; Yuichi Iwaki; John J. Fung; Thomas E. Starzl; Anthony J. Demetris
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 929 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
Twenty-two consecutive liver allograft recipients, who tested positive for immunoglobulin G (IgG) lymphocytotoxicity were subjected to pretransplantion and posttransplantation immunologic monitoring of antidonor IgG lymphocytotoxic antibody titers, total hemolytic complement activity (CHI,), circulating immune complexes (CIC), and platelet counts in an effort to improve our understanding of the preformed antibody state in clinical hepatic transplantation. Ten contemporaneous liver transplant recipients whose crossmatch results were negative and who experienced severe hepatocellular damage early after transplantation were included as controls. Crossmatch test results were negative 1 day after transplantation and during the 1 month follow-up remained negative in 14 of 22 (64%) sensitized recipients, most of whom had relatively low ( ~1 : 1 6 ) antidonor IgG antibody titers before transplantation. After transplantation, this group and the control group experienced no thrombocytopenia, no increase of CIC, and a gradual increase in CHl, activity that reached normal levels within 1 week. A strong negative correlation between prothrombin time (PT) and CHlW activity in these groups of patients suggested that changes in CHI, activity (P < .0005) were tightly linked to liver synthetic function. In contrast, the crossmatch test results remained positive after transplantation in 8 of 22 (36%) sensitized recipients, all of whom had relatively high (>1:32 to 1024) pretransplantation titers of anti-donor IgG antibodies. After transplantation these patients developed a syndrome that was characterized by decreased CHlm activity and increased CIC compared with pretrans-Abbreviations: IgG, immunoglobulin G; CIC, circulating immune complexes; AST, aspartate transaminase; CHioo, hemolytic complement activity; PGE, , prostaglandin El; PT, prothrombin time.