Immunogenicity of multiple antigen peptides (MAP) containing T and B cell epitopes of the repeat region of the P. falciparum circumsporozoite protein

✍ Scribed by Dona Yamuna Munesinghe; Pedro Clavijo; Mauricio Calvo Calle; Ruth S. Nussenzweig; Elizabeth Nardin

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 661 KB
• Volume: 21
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830211217

✦ Synopsis

Imunogenicity of multiple antigen peptides (MAP) containing T and B cell epitopes of the repeat region of the P. falciparum

circumsporozoite protein*

The immunogenicity of multiple antigen peptides (MAP) constructs containing T and B cell epitopes of the repeat region of the I! fakiparum circumsporozoite (CS) protein was examined in vitro, using a humanT cell clone, and in vivo, using four different strains of mice. All the MAP constructs that contained the Tcell epitope, (DPNANPNVDPNANPNV) , stimulated proliferation and interferon-y production by a humanT cell clone specific for this epitope which is located in the 5' end of the repeat region of the I! falciparum CS protein. These human T cells did not recognize MAP that contained only the B cell epitope, (NANP)3, which is located in the 3' repeat region. Optimal antibody responses were obtained in mice immunized with MAP containing four copies of tandemly arranged T and B cell epitopes, (TB)4. The murine immune response to the MAP constructs was genetically restricted. Mice of a high responder strain, C57BL, recognized both the 5' and 3' repeat sequences in the MAP as T, as well as B, cell epitopes and developed very high anti-MAP and anti-sporozoite antibody titers. A/J and C3H mice,which were intermediate responders, developed lower antibody titers which varied according to the orientation of theTvs. the B cell epitopes within the MAP constructs. BALB/c mice were nonresponders and did not develop antibodies following immunization with any of the MAP constructs containing the 5' and 3' repeats of the I? falciparum CS protein.