Immunogenetic analysis of microsomal and non-microsomal lipoproteins from normal and malignannt mouse tissues for histocompatibility-2 (H-2) antigens
✍ Scribed by Joy Palm; L. A. Manson
- Book ID
- 102881802
- Publisher
- John Wiley and Sons
- Year
- 1966
- Tongue
- English
- Weight
- 986 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
The H-2 antigenic properties of lipoprotein fractions from malignant (L-517SY leukemia) and normal (spleen, thymus, liver, kidney) mouse tissues have been studied by serological and immunological tests, and the results compared to the previously described activities of these fractions in homograft-sensitization tests. Although, in general, the relative activities in the different assays parallel each other some notable exceptions were found. The non-microsoma1 lipoprotejns from leukemic tissue, inactive in homograft-sensitization tests, did elicit H-2 antibody. Also, the liver microsomal lipoproteins, which are inactive in homograft-sensitization tests in amounts 400 X the minimal effective doses of spleen preparations, exhibited, in in witro agglutinin-inhibition tests, approximately one-fourth the H-2 activity of the latter. Other findings of note include the high antibody-eliciting potency of the spleen and leukemia microsomal lipoproteins (15 Fg protein was sufficient to initiate primary immunization and 1 pg protein to cause an anamnestic response); and the quantitive identity of H-2 antigen activity of the microsomal lipoproteins from spleen and thymus.