Immunofluorescent serum gliadin antibodies in children with coeliac disease and various malabsorptive disorders

✍ Scribed by M. Stern; K. Fischer; R. Grüttner

• Book ID: 104780042
• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 780 KB
• Volume: 130
• Category: Article
• ISSN: 0340-6997
• DOI: 10.1007/bf00455261

✦ Synopsis

An immunofluorescent gliadin antibody assay is described using pyruvic aldehyde-stabilized human erythrocytes coated with gliadin. Fifty coeliac children all had high serum IgG-antigliadin titres during a normal diet or a challenge with gluten. On a gluten-free diet (30 children), titres were much lower. In patients followed-up for one year on a gluten-free diet, an initial rise in titres was followed by a slow decline. On challenge, IgG-antigliadin titres showed a slow rise or persistence at the same level in most patients. Fifty-two percent of control children with malabosrptive disorders, but without the typical "flat" mucosal lesion on jejunal biopsy, were shown to have positive titres in their sera, as were 6% of normal children and 4% of adult blood donors. The fluorescent antibody technique was compared with methods commonly used to detect wheat-protein antibodies, and was found to be superior to all of them. The immunofluorescent gliadin antibody assay appears to be useful in following-up children with coeliac disease, and in selecting patients for jejunal biopsy, although it does not replace biopsy.