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Immunofluorescent localization of bovine acrosin

โœ Scribed by Garner, Duane L. ;Easton, Marlys P. ;Munson, Mark E. ;Doane, Mark A.

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
236 KB
Volume
191
Category
Article
ISSN
0022-104X

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โœฆ Synopsis

Abstract

Acrosin, a trypsinโ€like proteinase, was localized in the acrosome of methanolโ€fixed bovine spermatozoa by the indirect immunofluorescent technique. Antiโ€acrosin immunoglobulin was obtained from a rabbit immunized with highly purified bovine acrosin. The results of agaroseโ€gel immunodiffusion analyses indicated that although the antibody was specific for the acrosomal enzyme, it crossโ€reacted with ovine acrosin.

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