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Immunofluorescence localization of the regulatory subunit type II of cAMP-dependent protein kinase in PC12 and 3T3 cells in different proliferative states

✍ Scribed by Igor I. Shmyrev; Irina D. Grozdova; Aleksei D. Kondratyev; Elena G. Mamayeva; Evgenii S. Severin

Publisher: Springer
Year: 1990
Tongue: English
Weight: 398 KB
Volume: 93
Category: Article
ISSN: 0300-8177
DOI: 10.1007/bf00223491

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✦ Synopsis

Localization of the regulatory subunit of cAMP-dependent protein kinase type II was studied in proliferating and quiescent fibroblasts 3T3 and in a cell line of neural origin pheochromocytoma PC12. In actively proliferating PC12 cells the regulatory subunit was found to be localized in the nucleus. Transition of these cells into a quiescent state was accompanied by a regulatory subunit translocation to the cytoplasm. In 3T3 cells the regulatory subunit was localized in the cytoplasm both in the quiescent and proliferating (though less actively than PC12 cells) states. Similar results were obtained both with monoclonal antibodies and with rabbit monospecific antiserum raised against the regulatory subunit type II from pig brain.

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