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Immunofluorescence and anti-complement immunofluorescence absorption tests for quantitation of epstein-barr virus-associated antigens

✍ Scribed by Beverley M. Reedman; J. Hilgers; F. Hilgers; G. Klein

Book ID
102276413
Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
407 KB
Volume
15
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Immunofluorescence absorption methods are described which permit quantitative estimation and differentiation of Epstein‐Barr virus (EBV)‐associated antigens (virus capsid antigen, VCA, early antigen, EA and EBV‐determined nuclear antigen, EBNA) in cell extracts. EBNA was present in all cell lines (producer and non‐producer) which carried the EBV‐genome, while VCA and EA were present in producer lines only. All the antigens were absent from a lymphoid cell line (MOLT‐4) which lacked the EBV‐genome, as well as from leukemia cells from peripheral blood. The techniques demonstrated antigenic identity of the various antigens when prepared from different cell lines.

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