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Immunoelectron localization of mitochondrial F1 factor in cryosections of heart muscle cells

✍ Scribed by Real, Suzana Corte ;de Meirelles, Maria de Nazareth Leal

Publisher
Wiley (John Wiley & Sons)
Year
1991
Tongue
English
Weight
617 KB
Volume
18
Category
Article
ISSN
0741-0581

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✦ Synopsis

Abstract

Immunocytochemistry was used to investigate the localization of F1 ATPase in mitochondria of cryosections of adult mouse heart muscle cells. The initial aldehyde fixation was the only denaturation step for antigens. The fine structure was preserved with contrast enhancement as the sections were maintained hydrated, with the advantage that the entire procedure is completed in one working day. The reaction was highly specific, and entire mitochondria were labeled with the Protein A‐gold complex. A new analytical technique, electron spectroscopic imaging (ESI), contributed to a better visualization of the localization of the F1 factor.

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