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Immunocytochemical demonstration of glycogen phosphorylase in Müller (glial) cells of the mammalian retina

✍ Scribed by Dr. Brigitte Pfeiffer; Jens Grosche; Andreas Reichenbach; Bernd Hamprecht

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
692 KB
Volume
12
Category
Article
ISSN
0894-1491

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✦ Synopsis

Abstract

Glycogen phosphorylase (GP) was immunocytochemically detected in Müller cells of the rabbit and rat retina using a monoclonal antibody raised against bovine brain GP. Immunofluorescence and immunoenzymatic procedures were applied on isolated, Müller cells and sections of paraformaldehyde‐fixed, paraffin‐embedded retinas. All methods used revealed positive immunostaining. GP immunoreactivity was most intense in the Müller cell endfeet and the pericarya, corresponding to the nerve fibre layer and the inner nuclear layer in the retina. The presence of GP in Müller cells stresses the important role of these glial cells in the energy metabolism of the mammalian retina.

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