Immunocytochemical assay of estrogen receptors in endometrial carcinoma with monoclonal antibodies. Comparison with biochemical assay

✍ Scribed by Louis P. Pertschuk; Anne Marie Beddoe; Lester S. Gorelic; Sydney A. Shain

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 566 KB
• Volume: 57
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19860301)57:5<1000::aid-cncr2820570521>3.0.co;2-9

✦ Synopsis

Specimens of endometrial adenocarcinoma, surgically obtained from 18 women, were analyzed for distribution of estrogen receptors by an immunocytochemical assay, employing monoclonal anti-estrophilin antibodies and the peroxidase-antiperoxidase technique. Results were compared with biochemical receptor analyses, and were in concordance in 83% of them. Marked tumor cell and tissue receptor heterogeneity were apparent with the immunocytochemical method, and a variety of patterns of nuclear staining in positive tissue samples were revealed. These results indicate that the immunohistologic method will provide a number of entirely new variables that may eventually be correlated with both clinical and pathologic features of this malignancy, and may prove to be of value in the prediction of clinical endocrine response.

Cancer 57: 1000-1 004.1986.

HE DETECTION of specific receptor sites for steroid

T hormones in breast cancer, and the subsequent demonstration of a more favorable clinical response to endocrine manipulation in patients with receptor-positive tumors, laid the groundwork for the employment of progestational agents in the therapy of advanced endometrial carcinoma.' In this first study, of 21 women, one third were found to achieve an objective clinical response to moderate dosages of progestins. These initial findings have since been confirmed, and it now appears that tumor hormone response is more likely to occur in well-differentiated Subsequently, estrogen receptors (ER) and progesterone receptors (PgR) have been measured by biochemical procedures in endometrial carcinoma, and the results correlated with histologic differentiation, as well as with endocrine r e s p o n ~e . ~? ~ Although