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Immunochemiluminescent focus assays for the quantitation of hepatitis A virus and rotavirus in cell cultures

✍ Scribed by Gary P Richards; Michael A Watson

Book ID
114139648
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
753 KB
Volume
94
Category
Article
ISSN
0166-0934

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A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr