Production Of Polyclonal Antisera -- Efficient Elution Of Purified Proteins From Polyvinylidene Difluoride Membranes (immobilon) After Transfer From Sds-page And Their Use As Immunogens -- Raising Polyclonal Antibodies Using Nitrocellulose-bound Antigen -- Synthesis Of Peptides For Use As Immunogens -- Production And Characterization Of Antibodies Against Synthetic Peptides -- Preparation And Testing Of Monoclonal Antibodies To Recombinant Proteins -- Screening Of Monoclonal Antibodies Using Antigens Labeled With Acetylcholinesterase -- Purification Of Immunoglobulin G (igg) -- Epitope Mapping -- Enzyme-antienzyme Method For Immunohistochemistry -- Double Label Immunohistochemistry On Tissue Sections Using Alkaline Phosphatase And Peroxidase Conjugates -- Immunohistochemical Detection Of Bromodeoxyuriding-labeled Nuclei For In Vivo Cell Kinetic Studies -- Avidin-biotin Technology: Preparation Of Biotinylated Probes -- Avidin-biotin Technology: Preparation Of Avidin Conjugates --^ Immunochemical Applications Of Avidin-biotin Technology -- Preparation Of Gold Probes -- Immunogold Probes For Light Microscopy -- Immunogold Probes In Electron Microscopy -- Electron Microscopic Silver Enhancement For Double Labeling With Antibodies Raised In The Same Species -- Quantitative And Qualitative Immunoelectrophoresis -- Rocket Immunoelectrophoresis Techniques Or Electroimmunodiffusion -- Crossed Immunoelectrophoresis -- Crossed Immunoaffinoelectrophoresis -- Immunodetection Of Proteins By Western Blotting -- Erasable Western Blots -- Colloidal Gold Staining And Immunoprobing On The Same Western Blot -- Colloidal Gold Staining And Immunodetection In 2-d Protein Mapping -- Fluorescent Protein Staining On Nitrocellulose With Subsequent Immunodetection Of Antigen -- Competitive Elisa -- Twin-site Elisas For Fos And Myc Oncoproteins Using The Ampak System -- Preparation Of Cytotoxic Antibody-toxin Conjugates --^ Immunoaffinity Purification And Quantification Of Antibody-toxin Conjugates -- An Immuno-slot-blot Assay For Detection And Detection And Quantitation Of Alkyldeoxyguanosines In Dna -- Production Of Monoclonal Antibodies For The Detection Of Chemically Modified Dna -- Sensitive Competitive Enzyme-linked Immunoassay For Quantitation Of Modified Bases In Dna -- An Immunochemical Assay For Detecting Transition Of B-dna To Z-dna -- Cell Sorting Using Immunomagnetic Beads -- Cell Preparation For Flow Cytometry -- Preparation Of Rat Lung Cells For Flow Cytometry -- The Isolation Of Rat Hepatocytes For Flow Cytometry -- Flow Cytometric Analysis Of Cells Using An Antibody To A Surface Antigen -- Multiple Immunofluorescence Analysis Of Cells Using Flow Cytometry -- Cell Kinetic Studies Using A Monoclonal Antibody To Bromodeoxyuridine -- Production And Use On Nonradioactive Hybridization Probes -- Cellular Human And Viral Dna Detection By Nonisotopic In Situ Hybridization --^ Chromosomal Mapping Of Genes By Nonisotopic In Situ Hybridization -- Nonisotopic In Situ Hybridization -- Biotinylated Probes In Colony Hybridization -- Screening Of Agt11 Cdna Libraries Using Monoclonal Antibodies -- Expression Of Foreign Genes In Mammalian Cells Using An Antibody Fusion System. Edited By Margaret M. Manson. Includes Bibliographical References And Index.