๐”– Bobbio Scriptorium
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Immunochemical detection using the light-addressable potentiometric sensor

โœ Scribed by Peter R Panfili; Kilian Dill; John D Olson

Book ID
104361771
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
570 KB
Volume
5
Category
Article
ISSN
0958-1669

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โœฆ Synopsis

Rapid, sensitive and flexible assay systems are needed for immunoassays, receptor-ligand binding studies and DNA probe assays. Filtration capture and sensor detection offer several advantages to these areas. Although dependent on the affinity of the specific binders employed, the sensitivity of these techniques can be in the order of 10-12 M, and total assay time can be less than 15 minutes.

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โœ Kim R. Rogers; John C. Fernando; Roy G. Thompson; James J. Valdes; Mohyee E. Eld ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 700 KB

The nicotinic acetylcholine receptor, purified from Torpedo electric organ, was coupled to a light addressable potentiometric sensor (LAPS) to form a LAPS-receptor biosensor. Receptor-ligand complexes containing biotin and urease were captured on a biotinylated nitrocellulose membrane via a streptav