Immunochemical characterization of tumor-associated surface antigens on a moloney leukemia virus-lymphoma, MBL-2
✍ Scribed by Ah-Kau Ng; K. Robert McIntire; Seikichi Suzuki; Tadao Aoki; Ronald B. Herberman
- Book ID
- 102863514
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- French
- Weight
- 901 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
Tumor‐associated surface antigens (TASA)^4^ on a Moloney leukemia virus (M‐MuLV)‐induced lymphoma, MBL‐2, in C57BL/6 mice (B6) were characterized. The surface proteins of MBL‐2 cells were selectively radioiodinated and then extracted by Nonidet P40. The solubilized materials were then reacted with a variety of antisera: monospecific antisera to murine leukemia viral proteins (anti‐gp69/71, anti‐p30, anti‐p15, anti‐p12 and anti‐p10), sera from B6 which regressed murine sarcoma tumors induced by murine sarcoma virus (anti‐MSV) and a rabbit anti‐MBL‐2 antiserum. The resulting radioimmune precipitates were analyzed and compared in sodium dodecyl sulfate‐polyacryl‐amide gel electrophoresis (SDS‐PAGE). The following results were obtained. (1) Among all anti‐viral protein antisera tested only anti‐gp69/71 was active and detected a protein doublet of gp69/71 and its degradation fragments of 42,000 and 35,000 daltons. (2) Radioimmune precipitates prepared with anti‐MSV showed a SDS‐PAGE pattern similar to that seen with anti‐gp69/71. This result indicated that the surface antigen detected by the anti‐MSV serum on MBL‐2 tumor cell was probably a viral envelope antigen. (3) The rabbit anti‐MBL‐2 serum detected on the cell membrane an antigen of approximately 95,000 daltons which was tumor‐associated and did not appear to be related to virion components. The anti‐MBL‐2 serum still reacted with the 95,000 dalton antigen after absorption with disrupted M‐MuLV virus and with gp69/71 and p30 purified from the virus.