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Immunoassays of peptide hormones and their chemical aspects

✍ Scribed by L. Moroder; R. Nyfeler; M. Gemeiner; H. Kalbacher; E. Wüunsch


Publisher
Wiley (John Wiley & Sons)
Year
1983
Tongue
English
Weight
306 KB
Volume
22
Category
Article
ISSN
0006-3525

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✦ Synopsis


Peptidchemie, 0-8033 Martinsried bei Munchen, Federal Republic of Germany

Synopsis

The N-terminal maleoyl-0-alanyl derivative of human gastrin-[2-17] has been synthesized as a model compound to investigate the usefulness of such peptide derivatives for their mild and selective conjugation, via reaction with thiol groups, to high-molecular-weight carrier molecules to produce antigens, as well as to radioiodinable or fluorogenic molecules to prepare tracers for immunoassays. In this context the examined enzyme substrate-gastrin conjugate was found to exhibit the full immunoreactivity of the parent peptide and to be well suited as tracer in a gastrin fluorescence-immunoassay.


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