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Immunoassay and Activity of Calcium-Activated Neutral Proteinase (mCANP): Distribution in Soluble and Membrane-Associated Fractions in Human and Mouse Brain

✍ Scribed by Kiyoshi H. Takeuchi; Ken-Ichi Saito; Ralph A. Nixon


Book ID
111177684
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
821 KB
Volume
58
Category
Article
ISSN
0022-3042

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The activity of calcium-activated neutral proteinase (mM CANP) was determined in subcellular fractions of rat brain. The CANP activity in whole homogenate and its membrane fractions including myelin was increased tenfold following treatment with Triton X-100. The majority of the activity (60%) was i

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## Abstract Calcium‐activated neutral proteinase (CANP) activity was determined in cytosolic and membranous subcellular fractions of transformed Schwann cells (tSc). The ΞΌM and mM Ca^2+^ ‐sensitive (μ‐and mCANP) forms of CANP were separated by DEAE and phenyl Sepharose column chromatography, the la