Immunoalkaline phosphatase with cerium-based cytochemistry for double staining at the transmission electron microscopic level

✍ Scribed by Rowden, Geoffrey ;Dean, Shirley

• Publisher: Wiley (John Wiley & Sons)
• Year: 1991
• Tongue: English
• Weight: 758 KB
• Volume: 18
• Category: Article
• ISSN: 0741-0581
• DOI: 10.1002/jemt.1060180205

✦ Synopsis

Immunoelectron microscopic localization of surface antigens on lymphocytes is possible using alkaline phosphatase combined with cerium-based cytochemical methods. Distinctive electron-dense deposits are easily identified at sites of antibody binding. Mouse splenocytes showing surface immunoglobulin localization and human peripheral blood lymphocytes stained for the MHC-Class II antigen HLA-DR illustrate the results. Double staining for murine Ia antigen by the alkaline phosphatase procedure, combined with immunogold labeling of antigens identified on dendritic cells, i.e., NLDC-145, demonstrates the utility of the cerium cytochemical method.