✦ LIBER ✦

Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application

✍ Scribed by H. Yanagihara; R. Sakata; H. Minami; H. Tanaka; Y. Shoyama; H. Murakami

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 668 KB
Volume: 335
Category: Article
ISSN: 0003-2670
DOI: 10.1016/s0003-2670(96)00371-6

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✦ Synopsis

Immunoaffinity column chromatography using anti-forskolin monoclonal antibodies appears to be far superior to previously published separation methods based on crystallization and chromatography. The capacity of the inununoaffinity column as determined by ELISA is 9 pgml-'. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.