Immunoaffinity chromatographic method for the detection of pesticides

✍ Scribed by Boris B. Kim; Eugenii V. Vlasov; Peter Miethe; Alexey M. Egorov

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 558 KB
• Volume: 280
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85121-y

✦ Synopsis

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An immunoaffinity chromatographic (IC) method for the determination of 2,4-dichlorophenoxyacetic acid (2,4-D) and atrazine was developed. The sensitivity was 1 and 0.1 pg 1 -t for 2,4-D and atrazine, respectively, the relative standard deviation was in the range 3-8% and the total assay time per sample was 10 min. IC was combined with three detection systems: spectrophotometric, chemiluminescence and fluorescence. The whole procedure can be fully automated and applied to the quasicontinuous control of pesticide contamination and a rapid assay under out-of-laboratory conditions.