Immuno-physical properties of Pseudomonas aeruginosa protein-lipopolysaccharide complex treated with surfactants
✍ Scribed by Rubio, Nazario ;Portol�s, Antonio ;Lopez, Rubens
- Book ID
- 104766306
- Publisher
- Springer-Verlag
- Year
- 1973
- Weight
- 913 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0003-9276
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✦ Synopsis
A reversible dissociation of the protein-lipopolysaccharide complex (Pr-LPS) isolated from Pseudomona8 aeruginosa after sodium deoxycholate treatment was demonstrated by ultracentrifugation, gel filtration and immunological techniques. Removal of deoxyeholate from the antigen solution restored it to a molecular weight similar to the original substance. In addition, subunits of the antigen seem to retain the ability to precipitate with anti Pr-LPS serum.
Neither high concentrations of polymyxin B nor surfactants as Triton X-100 or Tween-80 produced this molecular dissociation in the complex.