Immuno-gel diffusion studies with the herpes type virus (HTV) associated with burkitt's lymphoma
✍ Scribed by Mitsuri Konn; David S. Yohn; Yorio Hinuma; Junji Yamaguchi; James T. Grace Jr.
- Publisher
- John Wiley and Sons
- Year
- 1969
- Tongue
- English
- Weight
- 723 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0008-543X
No coin nor oath required. For personal study only.
✦ Synopsis
Antisera produced in rabbits to the Herpes-type virus ( H T V ) found in the H R l -K subline of the P3-J Burkitt cell line yielded 3 predpitin lines by gel immunodiffusion (ID) specific for H T V antigens. Similar lines formed with h u m a n sera positive by indirect immunofluorescence (IF). Lines of identity were formed with rabbit and human antisera for 2 lines designated "a" and "b"; the former was farthest from the antigen well. The 3rd precipitin line, "c," was next to the antigen well and contained antibody coated intact virions. The precipitating antibodies could be absorbed with H T V containing cells but not with the HTV-free cells Raji. Of the 475 h u m a n sera tested, 79 yielded the #'a" line, 8 the "b" line and 2 the "c" line. A n IF titer of 1:lOO or greater was present in 78 of the 79 ID positive sera. I t was found that the greater the titer by IF the higher the proportion of sera that were positive by ID. This relationship was true whether the sera were from patients with malignant disease or nonmalignant disease. However, consistently a greater proportion of sera from patients with malignant disease were ID positive than sera from patients with nonmalignant disease, even though IF titers were identical. The factors accounting for this difference are not fully known.
NTIBODIES TO THE HERPES-TYPE
) associated with Burkitt lymphoma cells have been demonstrated in sera from Burkitt lymphoma patient& 6 , 6 , 8. 12, 16 from normal individualsl.
12016 and more recently in sera from infectious mononucleosis patients.?. 1 4 Generally, these antibodies have been demonstrated by indirect immunofluorescent tests with cells chronically infected with the virus.6 At least some of these antibodies From the Viral Oncology Department, Roswell Park Memorial Institute, Buffalo, N.Y.