Immunization with outer-membrane protein(s) ofBacteroides fragilis: an experimental study in mice

✍ Scribed by T. Khanna; D. Panigrahi; N. K. Ganguly; S. Majumdar

• Book ID: 104723834
• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 258 KB
• Volume: 10
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00327951

✦ Synopsis

A crude outer-membrane protein (OMP) preparation from a strain of Bacteroides fragilis, grown in supplemented brain-heart infusion broth, was tested for its protective effect against subcutaneous infection in mice. Immunization with six doses, each of 100, 150 or 200 μg OMP preparation, gave some protection: abscesses completely disappeared 15 to 22 days after immunization. In non-immunized animals and animals immunized with doses of 10, 20, 40 or 80 μg each, well demarcated abscesses were seen beyond day 22 post-immunization. Although crude OMP elicited good antibody response, with maximum titres on day 4 post-immunization, high titres could not be associated with healing of the abscesses.