Immunization with anti-idiotype monoclonal antibodies bearing the internal image of the renal-cell carcinoma-associated antigen G250 induces specific cellular immune responses
✍ Scribed by Hirotsugu Uemura; Eigoro Okajima; Frans M. J. Debruyne; Egbert Oosterwijk
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- French
- Weight
- 609 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
We have previously isolated and characterized 6 anti-idiotype antibodies (Ab2s) directed against monoclonal antibody G250 (MAbG250) which reacts with a tumor-associated antigen (TAA) expressed in a large proportion of human renal-cell carcinomas (RCC). These 6Ab2s (NUH31,51,71,82,91: IgG,, NUH44: IgGA showed MAbG250 binding site specificity and induction of anti-TAA antibody resembling MAbG250 (so-called Ab I ') in rabbits, indicating that they are internal image antibodies. To investigate whether these Ab2s could induce G250-TAAspecific cell-mediated immunity, delayed-type hypersensitivity (DTH) tests were carried out with G250 antigen-positive and/or -negative cells in the ears of BALB/c mice. Mice primed with Ab2 showed antigen-specific DTH responses, whereas no significant DTH response was observed with G25O-negative cells. This antigen-specific DTH could be transferred to naive mice by lymphocytes harvested from Ab2-sensitized mice. In addition to the classical DTH responses observed 24 and 48 hr after tumor challenge, an early-phase antigen-specific hypersensitivity response was seen 2 hr after challenge.