Immunity against moloney sarcoma virus in H-2Db mutant bm14 mice. Unimpaired tumor immunity despite absence of a virus-specific cytotoxic T-cell response
✍ Scribed by Marijke J. Stukart; Jolande Boes; Cornelis J. M. Melief
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- French
- Weight
- 501 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
It was shown previously that B6.C-H-Zbm" (bm14) mice, carrying a mutation in the H-2Db locus, are unable to generate cytotoxic T cells (CTL) against Moloney murine sarcoma virus (M-MSV). We now report an analysis of tumor induction and regression kinetics and of immunity to the virus, following the injection of graded doses of M-MSV into C57BU6 (B6) and bm 14 mice. Contrary to expectation, bm14 mice showed slightly less tumors than wildtype B6 mice. Moreover, all bm14 mice that developed a tumor were able to reject this tumor, even after injection of the highest virus dose tested. From the spleen cells of bm I4 mice that had rejected tumors, no secondary in vitro CTL responses could be generated, in contrast to strong CTL responses generated from B6 spleen cells. Although bm14 mice were unable to generate virus-specific CTL, they showed nomal antibody and T-cell proliferative responses against Moloney virus, suggesting an intact Thelper-cell function. It is concluded that in bm I4 mice, under the conditions tested, virus-specific CTL are not generated despite excellent tumor immunity. Therefore, this CTL response is not necessary for protection against M-MSV-induced tumors. Protection is likely to be mediated by a normal T-cell proliferative response.