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Immune responses to weakly immunogenic virally induced tumors I. Overcoming low responsiveness by priming mice with a syngeneic in vitro tumor line or allogeneic cross-reactive tumor

✍ Scribed by Naomi Galili; B. Devens; D. Naor; Susanne Becker; Eva Klein


Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
645 KB
Volume
8
Category
Article
ISSN
0014-2980

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✦ Synopsis


Abstract

This report describes model systems which show low primary in vitro syngeneic cytotoxic responses to a Moloney‐induced YAC tumor (syngeneic in A mice) and a Rauscher‐induced RBL5 tumor (syngeneic in C57BL/6 mice) and examines different approaches to overcome these defects.

Two major findings were obtained: (a) spleen cells from A mice, injected with tumor cells from an in vitro tumor line YAC‐1, derived from YACL, could generate a significant syngeneic cytotoxic response. In contrast, spleen cells from A mice injected with tumor cells from the in vivo tumor line failed to generate a syngeneic cytotoxic response. Thus, tumor cells from the in vitro line were more immunogeneic that those from the in vivo line. (b) Spleen cells from A mice which were injected with the crossreactive allogeneic tumor RBL5, could generate significant cytotoxic responses to the syngeneic tumors YAC and YAC‐1. Similarly, spleen cells from C57BL/6 mice injected with the cross‐reactive allogeneic tumor YAC‐1, could generate a significant cytotoxic response to the syngeneic tumor RBL5. Thus, cross‐reactive allogeneic tumors could stimulate syngeneic cytotoxicity. The theoretical and the practical implications of these studies are discussed.