Immune rejection mechanisms in murine leukemia. I. Timing of tumor cell rejection process relative to the development of humoral and cell-mediated cytotoxic immune responses
✍ Scribed by Richard P. Ciavarra; Geronimo Terres
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- French
- Weight
- 776 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Studies were undertaken to investigate the relationship between cell-mediated and humoral immune responses in the rejection of LIZIOIMTX-Rev (LR) leukemia cells in CDZF, mice. The anti-LRantibudy (humoral) response was defined by both ik cytotoxic antibody titer and isotype composition, assessed by a complement-dependent cytotoxicity assay and radioimmune assay, respectively. The cytotoxic thymus m d e r i v e d lymphocyte response in the spleen was quantitated by IzIl release by '"I-IUdR-labelled target cells. Analysis of the sera of tumor-bearing mice indicated that the LR leukemia cells elicited a wide spectrum of anti-tumor antibody isotypes. In mice that mounted a rejection response, the IgM anti-LR response was transient, while in those that did not, the IgM response persisted. Antibody titers for all isotypes remained low until the onset of LR rejection. A t that time, high titers of IgG anti-LR antibodies, predominantly of the IgG, subc l w . were detected in sera of tumor-free mice. Thus, the LR rejection response coincided best with the appearance of high titers of IgG, anti-LR antibodies. A single i.p. injec-