Immune Homeostasis: Methods and Protocols (Methods in Molecular Biology, 979)

✍ Scribed by Andrew L. Snow (editor), Michael J. Lenardo (editor)

• Publisher: Humana
• Year: 2013
• Tongue: English
• Leaves: 215
• Category: Library

✦ Synopsis

Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders.  Immune Homeostasis: Methods and Protocols focuses on experimental techniques for measuring and analyzing immune cell dynamics, with a particular emphasis on examining lymphocyte programmed cell death in different contexts.  With contributions by leading experts in the field, the collection examines detailed protocols for studying various pathways of apoptosis and necrosis in different types of hematopoietic cells, both in vitro and in vivo, methods for studying the maintenance of lymphocyte populations in the steady-state or following infectious challenges in both mice and humans, and technical insights into state-of-the-art genomics tools, among other topics.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Valuable and easy to use, Immune Homeostasis: Methods and Protocols serves as an important toolkit for basic and clinical scientists interested in examining various aspects of immune homeostasis in both normal and disease-related contexts in order to further study the dynamic processes that contribute to homeostasis of the immune system.

✦ Table of Contents

Immune Homeostasis
Preface
Contents
Contributors
Chapter 1: Quantitating Lymphocyte Programmed Cell Death In Vitro Using Simple Kill Assays
Chapter 2: Fluorescence-Activated Cell Sorting-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death in Activated, Primary Human T Cells
Chapter 3: Evaluation of IL-2-Withdrawal-Induced Apoptosis in Human T Lymphocytes
Chapter 4: Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multiparameter Fluorescence-Activated Cell
Sorting-Based Technique
Chapter 5: Visualization of Fas-Mediated Death-Inducing Signaling Complex Formation by Immunoprecipitation
Chapter 6: Analyses of Programmed Cell Death in Dendritic Cells
Chapter 7: Detection of Necrosis by Release of Lactate Dehydrogenase Activity
Chapter 8: Assessment of CD4 + and CD8 + T Cell Responses Using MHC Class I and II Tetramers
Chapter 9: Homeostatic Proliferation of Mature T Cells
Chapter 10: Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers
Chapter 11: Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis
Chapter 12: Molecular Measurement of T Cell Receptor Excision Circles
Chapter 13: Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis
Chapter 14: Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders
Chapter 15: Flow Cytometric Measurement of SLAM-Associated Protein and X-Linked Inhibitor of Apoptosis
Chapter 16: In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells
Index