Immune complex transfer two-site chemiluminescent immunoassay for serum growth hormone in alevin chum salmon
✍ Scribed by Fukada, Haruhisa ;Ban, Masatoshi ;Chiba, Hitoshi ;Hara, Akihiko
- Publisher
- John Wiley and Sons
- Year
- 1998
- Weight
- 123 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
An immune complex transfer two-site chemiluminescent immunoassay (CLIA) for salmon growth hormone (GH) was developed to measure serum GH in alevin chum salmon (Oncorhynchus keta) using a chemiluminescent acridinium ester as a label. The immune complex transfer method dramatically reduced non-speci®cally bound of acridinium esterlabelled antibody without a decrease in the speci®c binding. Consequently, we could detect lower levels of GH than achieved previously in a two-site CLIA for salmon GH. The detection limit of the assay was 7.8 fg/mL and the standard curve was linear up to 250 fg/mL. Coef®cients of variation were 2.2±7.7% within-assay and 5.3±9.1% between-assay. We have developed a highly sensitive and reproducible GH method and applied it to measurement of GH in alevin chum salmon.