Normal human fibroblasts (the OUMS-24 strain), derived from a 6-week-old human embryo, were transformed (into the OUMS-24F line) and immortalized by repeated treatments (59 times) with 4-nitroquinoline I -oxide (4NQO). Treatment began during primary culture and ended at the 5 I st population doublin
Immortalization of mutant p53-transfected human fibroblasts by treatment with either 4-nitroquinoline 1-oxide or x-rays
โ Scribed by Keiji Kino; Kazuo Fushimi; Chong Gao; Tomoko Shima; Koichiro Mihara; Masayoshi Namba
- Book ID
- 107427512
- Publisher
- Society for In Vitro Biology
- Year
- 1997
- Tongue
- English
- Weight
- 672 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1475-2689
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๐ SIMILAR VOLUMES
We introduced the mutant p53 zene (codon 273Ar9-His) into human fibroblasts (SUSM-I ceils) previously immortalized with 4-nitroquinoline I -oxide (4NQO) and obtained 2 clonal cell lines (SUSM-I /p53-I and SUSM-l/p53-6) expressing the mutant p53. Since the genetic background of SUSM-l/p53 is the same
IMMORTALIZED In vitro models of malignant transformation of human cells may provide considerable insight into the mechanisms of multistep carcinogenesis. It is well established that normal human cells must be immortalized before they can be malignantly transformed; however, they are stringently dest