✦ LIBER ✦
Immobilized rhodanese: Some aspects of anion inhibition kinetics and modulation by cations
✍ Scribed by Nok, Andrew J. ;Nasir, Shuaibu M. ;Sa'Adatu, Yusuf
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 439 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0887-2082
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✦ Synopsis
The rat liver rhodanese (thiosulphate: cyanide sulfurtransferase EC 2.6.1.1) has been immobilized on polyacrylamide gels. The immobilized enzyme had a pH optimum of 7.4 and Km values of 3.25 mM and 1.12 mM for S20g-and KCN, respectively. The enzyme was competitively inhibited by NaNO, and CH,COONa and noncompetitively by amyl-nitrite. A modulation of activity was observed in the presence of Ca2+, Zn2+, and Cu2+. The results are discussed in line with the detoxicating function of liver rhodanese.