𝔖 Bobbio Scriptorium
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Immobilized reagent for bioluminescent ecological monitoring

✍ Scribed by Esimbekova, Elena; Kratasyuk, Valentina


Book ID
123246025
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
77 KB
Volume
136
Category
Article
ISSN
0168-1656

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A bioluminescent assay for 12-Ξ±-hydroxy
✍ Juergen Schoelmerich; John E. Hinkley; Ian A. Macdonald; Alan F. Hofmann; Marlen πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 519 KB

A bioluminescent assay for 12-cr-hydroxy bile acids was developed using enzymes coimmobilized onto Sepharose 4B. The immobilized enzymes used were a bacterial 12-a-hydroxysteroid dehydrogenase, bacterial luciferase, and NADPHPMN oxidoreductase or bacterial diaphorase. The assay was specific for 12+h