## Two-dimensional analysis of membrane proteins with isoelectric focusing in immobilized pH gradients in the first dimension Two-dimensional electrophoresis with isoelectric focusing in immobilized pH gradients in the first dimension was applied to the fractionation of hydrophobic proteins from th
โฆ LIBER โฆ
Immobilized pH gradients as a first dimension in shotgun proteomics and analysis of the accuracy of pI predictability of peptides
โ Scribed by Benjamin J. Cargile; Dana L. Talley; James L. Stephenson Jr.
- Book ID
- 102183742
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 277 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0173-0835
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The proteome of the yeast Saccharomyces cerevisiae was analysed by two-dimensional (2D) polyacrylamide gel electrophoresis utilizing a non-linear immobilized pH gradient (3-10) in the first-dimensional separation. Cells were labelled by [ 35 S]methionine incorporation in the respiro-fermentative pha