Immobilized metal ion affinity electrophoresis: A study with several model proteins containing histidine
β Scribed by Hany Goubran-Botros; Elizabeth Nanak; Jamal Abdul Nour; Gerd Birkenmeir; Mookambeswaran A. Vijayalakshmi
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 843 KB
- Volume
- 597
- Category
- Article
- ISSN
- 1873-3778
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## Abstract Oligomerization can endow proteins with novel structural and catalytic properties. The native dimer of bovine seminal ribonucleases (BSβRNase) binds, melts and catalyses the hydrolysis of doubleβstranded ribonucleic acids 30βfold better than its pancreatic homologue, the monomeric RNase
## Immobilized metal ion affinity chromatography (IMAC) is now a widely accepted technique for the purification of natural and recombinant therapeutic products and is beginning to find industrial applications. The design, optimization, and scale-up of a chromatographic process using IMAC demands a