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Immobilized enzymes as chromatographic phases for HPLC: The chromatography of free and derivatized amino acids on immobilized trypsin

โœ Scribed by Sylvie Thelohan; Ph. Jadaud; I. W. Wainer


Book ID
112719815
Publisher
Springer
Year
1989
Tongue
English
Weight
398 KB
Volume
28
Category
Article
ISSN
0009-5893

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Enzyme-based high-performance liquid chr
โœ Wai-Keung Chui; Irving W. Wainer ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 939 KB

Immobilized artificial membrane (IAM) HPLC supports have been used to immobilize the enzymes alpha-chymotrypsin and trypsin. The enzymes were trapped in hydrophobic cavities on the support and were not covalently attached to the IAM surface. The resulting IAM-enzyme supports retained the hydrolytic