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Immobilized Enzyme Particles Prepared by Radiation Polymerization of Polyurethane Prepolymer

✍ Scribed by Minoru Kumakura; Isao Kaetsu

Publisher
John Wiley and Sons
Year
1983
Tongue
German
Weight
658 KB
Volume
66
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

The immobilization of enzymes such as cellulase by radiation polymerization of dispersed polyurethane prepolymer was studied using tolylene‐2,4‐diisocyanate and 2‐hydroxyethyl methacrylate. The polyurethane particles were obtained by the dispersion of polyurethane prepolymer followed by radiation polymerization, in which the enzyme was immobilized on its surface by convalent bonding. The particle diameter of immobilized enzyme particles varied with monomer concentration and composition. The enzymatic activity of immobilized enzyme particles varied with the temperature of dispersion and irradiation, and decreased with increasing particle diameter.

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✍ Kaetsu, Isao ;Kumakura, Minoru ;Asano, Masaharu ;Yamada, Akio ;Sakurai, Yasuhisa πŸ“‚ Article πŸ“… 1980 πŸ› John Wiley and Sons 🌐 English βš– 720 KB

## Abstract The immobilization of some medically useful enzymes were studied by means of radiation‐induced polymerization at βˆ’78Β°C. Glucose oxidase and glucose peroxidase were immobilized in the form of thin membranes inside polyvinyl chloride tubes and on polyethylene films; these membranes showed