Immobilized enzymatic fluorescence capillary biosensor for determination of sulfated bile acid in urine
β Scribed by Yong-Sheng Li; Wei-Ping Liu; Xiu-Feng Gao; Dan-Dan Chen; Wei-Guang Li
- Book ID
- 108071956
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 396 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0956-5663
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## Multistep enzymatic reactions take place when sulfated bile acids (SBAs) pass through an immobilized enzyme reactor. First, SBAs take place in the reaction of desulfation under catalytical action of a bile salt sulfatase immobilized in the reactor and formed 3b-hydroxyl bile acids. Formed 3b-hydr
## Abstract A CZE with indirect LIF detection method was used for the determination of uric acid (UA) in human urine. UA and its coexisting analytes (__i.e__. hypoxanthine, xanthine and ascorbic acid) could be well separated within 4.5βmin at a voltage of 25βkV with 25Β°C cartridge temperature in a