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Immobilization on chitosan of a thermophilic βglycosidase expressed inSaccharomyces cerevisiae

✍ Scribed by Sabato D’Auria; Fernando Pellino; Francesco La Cara; Roberto Barone; Mosè Rossi; Roberto Nucci


Book ID
112892927
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
511 KB
Volume
61
Category
Article
ISSN
0273-2289

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## Abstract β‐Galactosidase was immobilized on chitosan using tris(hydroxymethyl)phosphine (THP) as a coupling agent to produce galactooligosaccharides (GOS) from lactose. Both the THP‐immobilized and the free enzymes were maximally achieved at pH 5.0 and the optimal temperature was 55 °C. The resi