B-Galactosidase was immobilized in a crosslinked poly(N-isopropylacrylamide-coacrylamide) hydrogel which exhibits an LCST(lower critical solution temperature) behavior. The hydrogel collapses above the LCST, and expands below the LCST. The temperature-dependent phase transition was around 37OC. The
Immobilization ofColeus blumeiplant cells in temperature-sensitive hydrogel
โ Scribed by Jun Han; Blanca C. Martinez; R. Roger Ruan
- Publisher
- Springer-Verlag
- Year
- 1996
- Tongue
- English
- Weight
- 290 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0951-208X
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## Abstract Three different temperature sensitive mutants derived from the Syrian hamster cell line BHK 21 were found to have greatly reduced DNA synthesis at the nonโpermissive temperature. These mutants are distinct by complementation analysis and behave at the nonโpermissive temperature as cell