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Immobilization of β-Galactosidase on Chitosan

✍ Scribed by Carlos R. Carrara; Amelia C. Rubiolo

Book ID
114420605
Publisher
American Institute of Chemical Engineers
Year
1994
Tongue
English
Weight
539 KB
Volume
10
Category
Article
ISSN
8756-7938

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Application of tris(hydroxymethyl)phosph
Application of tris(hydroxymethyl)phosphine as a coupling agent for β-galactosidase immobilized on chitosan to produce galactooligosaccharides
✍ Tzu-Chien Cheng; Kow-Jen Duan; Dey-Chyi Sheu 📂 Article 📅 2006 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 96 KB

## Abstract β‐Galactosidase was immobilized on chitosan using tris(hydroxymethyl)phosphine (THP) as a coupling agent to produce galactooligosaccharides (GOS) from lactose. Both the THP‐immobilized and the free enzymes were maximally achieved at pH 5.0 and the optimal temperature was 55 °C. The resi