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Immobilization of streptavidin for immunosensors on nanostructured surfaces

✍ Scribed by S. Kossek; C. Padeste; L. Tiefenauer

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
251 KB
Volume
9
Category
Article
ISSN
0952-3499

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✦ Synopsis

A modular immunosensor device based on silicon technology with electrochemical detection is presented. The sensor surface is functionalized with the antigen estradiol using the streptavidin-biotin system. The immobilized antigen is recognized by ferrocene-labeled antibody molecules in a competitive assay format. The ferrocene redox centers can be detected electrochemically. In order to facilitate the electron transfer, a gold layer structured in nanometer dimensions is used as an electrode. The functionslued silicon chip will be integrated into a micromachined fluid transport system. The resulting immunosensor device for estradiol represents a prototype allowing easy adaptation to other biotinylated analytes since the immobilized streptavidin serves as a universal anchor.

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Covalent immobilization of immunoglobuli
Covalent immobilization of immunoglobulin on a wafer surface for immunosensor bioselective matrix construction
✍ V.N. Zaitsev; L.I. Colomiets; A.V. Elskaya; V.V. Skopenko; J. Evans πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 547 KB

A hstrnct lmmunoglobulin G was immobilized on a wafer surface both covalently and adsorptively. For the preparation of a monolayer of covalently bonded groups, silylation with 3-aminopropyltriethoxysilane was carried out in anhydrous toluene with subsequent activation with glutaraldehyde solution.