Immobilization of Purified Penicillin Acylase in a Polarized Ultrafiltration Membrane Reactor
✍ Scribed by FRANCESCO M. VERONESE; ENRICO BOCcù; ODDONE SCHIAVON; GUIDO GRECO JR.; LILIANA GIANFREDA
- Book ID
- 118721887
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 166 KB
- Volume
- 434
- Category
- Article
- ISSN
- 0890-6564
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## Abstract The kinetic parameters for penicillin G hydrolysis in systems with penicillin G acylase from __Escherichia coli__ (free and immobilized on activated chitosan microbeads produced by electrostatic extrusion) were determined. The obtained kinetic results indicated that both systems (free a
The possibility of using the multicompartment immobilized enzyme reactor (MIER) in presence of a charged substrate is here explored. Penicillin G acylase is used to convert penicillin G (a free acid, with a pK of 2.6) into two charged products: phenyl acetic acid (PAA, with a pK of 4.2) and 6-aminop