𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Immobilization of proteases in composite hydrogel based on poly(N-vinyl caprolactam)

✍ Scribed by E. A. Markvicheva; A. S. Bronin; N. E. Kudryavtseva; L. D. Rumsh; Yu. E. Kirsh; V. P. Zubov

Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
433 KB
Volume
8
Category
Article
ISSN
0951-208X

No coin nor oath required. For personal study only.

✦ Synopsis

A novel one-step method was developed for entrapment of proteolytic enzymes, namely carborypeptidase B and trypsin, in polymer composite gel based on poly(N-vinyl caprolactam). Natie proteases and enzymes previously stabked by covalent attachment to poiy(vlnylpyrroUdone-co-acrokln) were lmmobked Ln gel% After lmmobiUzaUon, about 90% and 75% of origlnal trypsin and CPB actlvltles, respectlveiy, were retained. The Immobilized enzymes were active wlthin a wide pH range. The optimum temperature of the entrapped enzymes was approrlmately 25OC ldgber than that of tbe sohtble enzymes. The entrapped enzymes were successfully used to obtain human Insulin from recombinant prolnsulin.

πŸ“œ SIMILAR VOLUMES

An amperometric Meldola Blue-mediated se
An amperometric Meldola Blue-mediated sensor high sensitive to hydrogen peroxide based on immobilization of horseradish peroxidase in a composite membrane of regenerated silk fibroin and poly(vinyl alcohol)
✍ Haiying Liu; Xiaolin Zhang; Jianjun Wei; Xinxin Wu; Deyao Qi; Yongcheng Liu; Mia πŸ“‚ Article πŸ“… 1996 πŸ› Elsevier Science 🌐 English βš– 574 KB

A new composite membrane of poly(viny1 alcohol) (PVA) and regenerated silk fibroin (RSF) was successfully employed to immobilize horseradish peroxidase (HRP) and infrared (IR) was used to get insight in the structure of the composite membrane. An amperometric HRP-based sensor highly sensitive to hyd

The effect of gel composition on the ura
The effect of gel composition on the uranyl ions adsorption capacity of poly(N-vinyl 2-pyrrolidone-g-citric acid) hydrogels prepared by gamma rays
✍ Tuncer Γ‡aykara; Sinan Γ–ren; Γ–mer Kantoglu; Olgun GΓΌven πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 174 KB πŸ‘ 3 views

Poly(N-vinyl 2-pyrrolidone-g-citric acid) (PVP-g-CA) hydrogels with varying compositions were prepared from ternary mixtures of N-vinyl 2-pyrrolidone-citric acid-water by using 60 Co β₯-rays. The effect of gel composition on the uranyl ions adsorption capacity of PVP-g-CA hydrogels was investigated.