Immobilization of multi-enzyme in porous solid supports—a theoretical study
✍ Scribed by Md.M. Hossain; D.D. Do
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 982 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0009-2509
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✦ Synopsis
A theoretical study is presented dealing with the immobilization of two enzymes into a porous solid support. The developed model basically takes into account the interplay between restricted diffusions of two enzymes within intraparticle void space and their competitive adsorptions onto available functional sites on the interior surfaces. Simulations have shown that parameters such as binding rates of enzymes, initial bulk concentration ratio, pellet size and impregnating time have significant influence on the eventual distribution of two enzymes within the porous solid supports.
📜 SIMILAR VOLUMES
The total and active immobilized enzyme (IME) distributions in porous supports are studied both theoretically and experimentally. In order to determine experimentally the enzyme distribution profiles within a single particle, we construct a diffusion cell containing controlled-pore glass particles s
Clark et al. (1985) recently formulated a kinetic model for coupling restricted diffusion and immobilized reaction of enzyme molecules in a cylindrical pore using the concept of "pore central core restricted diffusion." This model takes into consideration the increase of diffusion resistance by t