Immobilization of invertase on polyethylenimine-coated cotton cloth

✍ Scribed by Hiroshi Yamazaki; Richard K. H. Cheok; Ann D. E. Fraser

• Book ID: 104632259
• Publisher: Springer Netherlands
• Year: 1984
• Tongue: English
• Weight: 274 KB
• Volume: 6
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00127033

✦ Synopsis

A simple, inexpensive and safe method for immobilizing yeast invertase was developed. Cotton flannel was coated with polyethylenimine.

Invertase was adsorbed to this cloth and was fixed by glutaraldehyde treatment. A column packed with cloth segments exhibited good flow and efficient sucrose hydrolysis over 3 months.